Composite

Part:BBa_K3046030:Design

Designed by: Philip Srensen   Group: iGEM19_DTU-Denmark   (2019-10-21)


pGIA2P1 insert


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 2116
    Illegal EcoRI site found at 3552
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 2147
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 2954
    Illegal PstI site found at 4696
    Illegal PstI site found at 5297
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 2116
    Illegal EcoRI site found at 3552
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 2954
    Illegal PstI site found at 4696
    Illegal PstI site found at 5297
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 2116
    Illegal EcoRI site found at 3552
    Illegal BglII site found at 4794
    Illegal BamHI site found at 1729
    Illegal BamHI site found at 2141
    Illegal BamHI site found at 2692
    Illegal XhoI site found at 548
    Illegal XhoI site found at 735
    Illegal XhoI site found at 963
    Illegal XhoI site found at 2552
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 2116
    Illegal EcoRI site found at 3552
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 2147
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 2954
    Illegal PstI site found at 4696
    Illegal PstI site found at 5297
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 2116
    Illegal EcoRI site found at 3552
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 2147
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 2954
    Illegal PstI site found at 4696
    Illegal PstI site found at 5297
    Illegal NgoMIV site found at 4684
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5185
    Illegal SapI site found at 5494


Design Notes

This plasmid was meant as a proof-of-concept for genome integration using the IS2 parts. None of the parts have therefore been domesticated to follow assembly standards.


Source

Most of the parts used to assemble this composite part was isolated by PCR amplification from genomic DNA from A. niger.

References